Rat corticotropin releasing hormone,CRH ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08038r

Sensitivity

1.25 ng/mL

Detection Range

1.25 ng/mL-20 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Competitive

Sample Volume

50-100ul

Alternative Names

corticotropin releasing hormone, CRF, corticotropin-releasing factor

Recovery

The recovery of human AGEs spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	97	92-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human AGEs in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-89
1:2	Average %	90
	Range %	85-95
1:4	Average %	94
	Range %	90-99
1:8	Average %	104
	Range %	100-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P01143
Tissue Specificity	Produced by the hypothalamus.

Applications	ELISA
Research Area	CRH

Storage	Store at 2-8°C. Please refer to protocol.

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