Rat Creatine Kinase MB isoenzyme,CK-MB ELISA Kit

Rat Creatine Kinase MB isoenzyme,CK-MB ELISA Kit

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Mfr.No.

CSB-E14403r

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

Creatine Kinase MB isoenzyme,CK-MB

Recovery

The recovery of mouse OTC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	97-109
EDTA plasma (n=4)	96	91-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse OTC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-102
1:2	Average %	91
	Range %	86-97
1:4	Average %	90
	Range %	85-94
1:8	Average %	94
	Range %	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
200	2.348	2.272	2.310	2.196
100	1.826	1.732	1.779	1.665
50	1.176	1.239	1.208	1.094
25	0.708	0.743	0.726	0.612
12.5	0.409	0.431	0.420	0.306
6.25	0.257	0.271	0.264	0.150
3.12	0.199	0.204	0.202	0.088
0	0.115	0.112	0.114

Species

Rattus norvegicus

Sample Type

Serum, Tissue homogenates, Urine, Plasma

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	CK-MB

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Upregulation of Cytoprotective Defense Mechanisms and Hypoxia-Responsive Proteins Imparts Tolerance to Acute Hypobaric Hypoxia Jain K et al,High Alt Med Biol,2013 Mar;14(1):65-77. Cardioprotective effects of Ficus religiosa in neonatal streptozotocin-induced diabetic cardiomyopathy in rats Raman Sharma et al,Biomedicine & Aging Pathology,2014 4(1) The clinical significance of circulating soluble RAGE in patients with severeSepsis Matsumoto H. et al,J Trauma Acute Care Surg. ,2015 Jun;78(6):1086-93; discussion 1093-4. CARDIOPROTECTIVE EFFECT OF TERMINALIA CHEBULA INTYPE II DIABETES INDUCED CARDIOMYOPATHY IN RATS Sharma R.et al,WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES,Volume 4, Issue 10, 2151-2163 Protective effect of low dose gadolinium chloride against isoproterenol-induced myocardial injury in rat Zheng YY. et al,Apoptosis.,2015 Sep;20(9):1164-75 Pretreatment with low-dose gadolinium chloride attenuates myocardial ischemia/reperfusion injury in rats Chen M.et al,Acta Pharmacol Sin.,2016 37(4):453-62. Enhanced hypoxic tolerance by Seabuckthorn is due to upregulation of HIF-1伪 and attenuation of ER stress Kanika Jain.et al,Applied Biomedicine,2016 Rho-kinase signaling pathway promotes the expression of PARP to accelerate cardiomyocyte apoptosis in ischemia/reperfusion Bian H.et al,Mol Med Rep,Jun 21 Cardioprotective effects of combined therapy with diltiazem and superoxide dismutase on myocardial ischemia-reperfusion injury in rats Chen C.et al,Life Sci,Jun 27 Propofol Through Upregulating Caveolin-3 Attenuates Post-Hypoxic Mitochondrial Damage and Cell Death in H9C2 Cardiomyocytes During Hyperglycemia Deng F.et al,Cell Physiol Biochem,Nov 9 Anti-atherosclerosis and cardio-protective effects of the Angong Niuhuang Pill on a high fat and vitamin D3 induced rodent model of atherosclerosis Wen-Juan Fu.et al,J Ethnopharmacol. ,Jan 4;195:118-126 Involvement of brain natriuretic peptide signaling pathway in the cardioprotective action of sitagliptin Samah S.Abbas.et al,Pharmacological Reports,Feb 7 Network pharmacology study reveals energy metabolism and apoptosis pathways-mediated cardioprotective effects of SHENQI FUZHENG Jie Liao .et al,Journal of Ethnopharmacology,August 23 Astragaloside IV Protects Rat Cardiomyocytes from Hypoxia-Induced Injury by Down-Regulation of miR-23a and miR-92a Gong L.et al,Cell Physiol Biochem,September 26

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.