Rat Delta-aminolevulinic Acid Dehydratase

Mfr.No.

E1184Ra

Sensitivity

0.53ng/ml

Detection Range

1-70ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Delta-aminolevulinic acid dehydratase (also known as ALAD) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

ALAD;ALADH;Delta-aminolevulinic acid dehydratase;Porphobilinogen Synthase

Molecular Weight

36032

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E1184Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	26.8	0.96	3.6
Intra-Assay	2	18	14.6	0.58	4
Intra-Assay	3	18	7.5	0.55	7.3

Background

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).

Protocol

These standard curves of E1184Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40ng/ml	2.642	2.511	2.472
	2.38
20ng/ml	1.45	1.391	1.352
	1.332
10ng/ml	0.869	0.859	0.82
	0.85
5ng/ml	0.426	0.456	0.416
	0.485
2.5ng/ml	0.27	0.278	0.239
	0.286
0ng/ml	0.033	0.039	0
	0.045

Gene ID	25374
UniProt ID	P06214

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112430	E1184Ra	48T	$320		Add to Cart
BHE12112430	E1184Ra	96T	$458		Add to Cart

Rat Delta-aminolevulinic Acid Dehydratase - ALAD ELISA Kit

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