Rat glial fibrillary acidic protein,GFAP ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08602r

Sensitivity

19.5 pg/mL

Detection Range

78 pg/mL-5000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

glial fibrillary acidic protein, FLJ45472

Recovery

The recovery of human DHEA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-102
EDTA plasma (n=4)	96	92-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human DHEA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-110
1:2	Average %	93
	Range %	87-100
1:4	Average %	95
	Range %	89-104
1:8	Average %	90
	Range %	85-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P47819
Tissue Specificity	Expressed in the cortex and hippocampus. Expression decreases following acute and chronic corticosterone treatment.

Applications	ELISA
Research Area	GFAP

Storage	Store at 2-8°C. Please refer to protocol.

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