Rat Glutaminase Kidney Isoform - Mitochondria

Mfr.No.

E1984Ra

Sensitivity

14.34ng/L

Detection Range

15-3000ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

GLS;Glutaminase kidney isoform, mitochondrial;Glutaminase kidney isoform, mitochondrial 65 kDa chain;Glutaminase kidney isoform, mitochondrial 68 kDa chain;K-glutaminase;L-glutamine amidohydrolase

Molecular Weight

74024

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E1984Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	403.8	22.32	5.5
Intra-Assay	2	18	925.1	36.38	3.9
Intra-Assay	3	18	584	29.61	5.1

Background

Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain.

Protocol

These standard curves of E1984Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.297	2.339	2.288
	2.382
800ng/L	1.45	1.392	1.341
	1.334
400ng/L	0.709	0.74	0.688
	0.77
200ng/L	0.423	0.425	0.374
	0.427
100ng/L	0.18	0.193	0.142
	0.206
0ng/L	0.049	0.051	0
	0.054

Gene ID	24398
UniProt ID	P13264

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113183	E1984Ra	48T	$320		Add to Cart
BHE12113183	E1984Ra	96T	$458		Add to Cart

Rat Glutaminase Kidney Isoform - Mitochondria - GLS ELISA Kit

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