Rat Kidney injury molecule 1,Kim-1 ELISA Kit

Rat Kidney injury molecule 1,Kim-1 ELISA Kit

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Mfr.No.

CSB-E08808r

Sensitivity

0.228 ng/mL

Detection Range

0.313 ng/mL-20 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

hepatitis A virus cellular receptor 1, HAVCR, HAVCR-1, KIM-1, KIM1, TIM-1, TIM1, TIMD1, T cell immunoglobin domain and mucin domain protein 1, kidney injury molecule 1

Recovery

The recovery of pig PYY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-101
EDTA plasma (n=4)	94	89-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig PYY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-99
1:2	Average %	96
	Range %	91-100
1:4	Average %	94
	Range %	89-98
1:8	Average %	93
	Range %	87-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1000	0.171	0.175	0.173
500	0.352	0.353	0.353
250	0.681	0.676	0.679
125	1.269	1.272	1.271
62.5	2.858	2.867	2.863

Species

Rattus norvegicus

Sample Type

Serum, Urine, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O54947
Tissue Specificity	Expressed at a low level in normal kidney but are increased dramatically in postischemic kidney. Expressed in proliferating bromodeoxyuridine-positive and dedifferentiated vimentin-positive epithelial cells in regenerating proximal tubules.

Applications	ELISA
Research Area	Kim-1

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Preservation of kidney function with combined inhibition of NADPH oxidase and angiotensin-converting enzyme in diabetic nephropathy Thallas-Bonke V. et al,American Journal of Nephrology,2010 32(1):73-82. Camel milk attenuates methotrexate-induced kidney injury via activation of PI3K/Akt/eNOS signaling and intervention with oxidative aberrations Hany H. Arab.et al,Food Funct,18 April Inhibition of Autophagy Attenuated Ethylene Glycol Induced Crystals Deposition and Renal Injury in a Rat Model of Nephrolithiasis Liu Y.et al,Kidney Blood Press Res,Feb 22 miR-182 enhances acute kidney injury by promoting apoptosis involving the targeting and regulation of TCF7L2/Wnt/B-catenins pathway HuicongLi.et al,European Journal of Pharmacology,May 4 Wnt4 is significantly upregulated during the early phases of cisplatin-induced acute kidney injury Yi-Xin He.et al,Scientific Reports,July 12

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SKU	Supplier No.	Size	Your price
BHE10508253	CSB-E08808r	96T	$495	Add to Cart
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BHE10508253	CSB-E08808r	10x96T	$4702.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.