Rat Pancreatic Lipase,PL ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E13784r

Alternative Names

pancreatic lipase, PL, pancreatic triacylglycerol lipase, triacylglycerol acylhydrolase

Reactivity

Rat

Detection Range

0.312 U/mL-20 U/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human SRC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	89	82-93

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SRC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-92
1:2	Average %	94
	Range %	86-98
1:4	Average %	89
	Range %	82-93
1:8	Average %	88
	Range %	82-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Sensitivity

0.078 U/mL

Limit Of Detection

450 nm

UniProt ID	P27657
Antigen	Pancreatic Lipase

Applications

ELISA

Research Area

PNLIP

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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