Rat Pancreatic Lipase,PL ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E13784r

Sensitivity

0.078 U/mL

Detection Range

0.312 U/mL-20 U/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

pancreatic lipase, PL, pancreatic triacylglycerol lipase, triacylglycerol acylhydrolase

Recovery

The recovery of human SRC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	89	82-93

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SRC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-92
1:2	Average %	94
	Range %	86-98
1:4	Average %	89
	Range %	82-93
1:8	Average %	88
	Range %	82-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Sample Type

Serum, Cell culture supernatants, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P27657
Tissue Specificity	Pancreatic secretory (zymogen) granule.

Applications	ELISA
Research Area	PNLIP

Storage	Store at 2-8°C. Please refer to protocol.

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