Rat Phospholipase D1 - PLD1 ELISA Kit

Mfr.No.

E1463Ra

Sensitivity

0.27ng/ml

Detection Range

0.5-200ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Phospholipase D1 (also known as PLD1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Choline phosphatase 1;Phosphatidylcholine-hydrolyzing phospholipase D1;Phospholipase D1;PLD 1;PLD1;rPLD1

Molecular Weight

123814

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E1463Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	31.4	2.02	6.4
Intra-Assay	2	18	62.5	2.55	4.1
Intra-Assay	3	18	12	0.73	6.1

Background

Function as phospholipase selective for phosphatidylcholine (By similarity). Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic (By similarity).

Protocol

These standard curves of E1463Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/ml	2.34	2.361	2.315
	2.383
60ng/ml	1.818	1.764	1.717
	1.709
30ng/ml	1.136	1.204	1.158
	1.272
15ng/ml	0.746	0.72	0.674
	0.694
7.5ng/ml	0.405	0.397	0.351
	0.388
0ng/ml	0.059	0.046	0
	0.033

Gene ID	25096
UniProt ID	P70496

Research Area	Others

Storage	2-8°C

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE12112695	E1463Ra	48T	$320		Add to Cart
BHE12112695	E1463Ra	96T	$458		Add to Cart

Rat Phospholipase D1 - PLD1 ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Rat Phospholipase D1 - PLD1 ELISA Kit

Rate it now..!!