Rat Protein S100-A8(S100A8) ELISA kit

Rat Protein S100-A8(S100A8) ELISA kit

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Mfr.No.

CSB-EL020641RA

Sensitivity

0.78 ng/mL

Detection Range

3.12 ng/mL-200 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

S100 calcium binding protein A8, 60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, MA387, MIF, MRP8, NIF, P8, OTTHUMP00000015330, S100 calcium-binding protein A8, S100 calcium-binding protein A8 (calgranulin A), calgranulin A, cystic fibrosis ant

Recovery

The recovery of human IGF-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-104
EDTA plasma (n=4)	97	90-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IGF-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:200	Average %	84
	Range %	80-92
1:400	Average %	97
	Range %	91-105
1:800	Average %	97
	Range %	92-104
1:1600	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.789	2.769	2.779	2.673
2000	2.034	2.046	2.040	1.934
1000	1.518	1.478	1.498	1.392
500	1.205	1.206	1.206	1.100
250	0.823	0.839	0.831	0.725
125	0.536	0.546	0.541	0.435
62.5	0.349	0.369	0.359	0.253
0	0.102	0.110	0.106	?

Species

Rattus norvegicus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P50115

Applications	ELISA
Research Area	S100A8

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508556	CSB-EL020641RA	96T	$695	Add to Cart
BHE10508556	CSB-EL020641RA	5x96T	$3336	Add to Cart
BHE10508556	CSB-EL020641RA	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.