Rat Sialate O-Acetylesterase

Mfr.No.

E2255Ra

Sensitivity

5.34ng/L

Detection Range

10-2000ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

RGD1310431;SIAE;Sialate O-acetylesterase;Sialate O-acetylesterase large subunit);Sialate O-acetylesterase small subunit;Sialic acid-specific 9-O-acetylesterase;Yolk sac protein 2

Molecular Weight

60466

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E2255Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	850.57	37.26	4.38
Intra-Assay	2	18	489.76	29.07	5.94
Intra-Assay	3	18	156.49	8.71	5.57

Background

Catalyzes the removal of O-acetyl ester groups from position 9 of the parent sialic acid, N-acetylneuraminic acid.

Protocol

These standard curves of E2255Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.023	2.124	2.071
	2.224
600ng/L	1.4	1.447	1.394
	1.493
300ng/L	0.722	0.724	0.671
	0.725
150ng/L	0.434	0.438	0.386
	0.443
75ng/L	0.289	0.284	0.232
	0.279
0ng/L	0.046	0.052	0
	0.058

Gene ID	363045
UniProt ID	P82450

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113387	E2255Ra	48T	$320		Add to Cart
BHE12113387	E2255Ra	96T	$458		Add to Cart

Rat Sialate O-Acetylesterase - SIAE ELISA Kit

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