Rat Soluble Lectin-like Oxidized Low Density Lipoprotein Receptor-1(sLOX-1)ELISA Kit

Rat Soluble Lectin-like Oxidized Low Density Lipoprotein Receptor-1(sLOX-1)ELISA Kit

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Mfr.No.

CSB-E15845r

Alternative Names

oxidized low density lipoprotein (lectin-like) receptor 1, CLEC8A, LOX1, SCARE1, lectin-type oxidized LDL receptor 1, oxidised low density lipoprotein (lectin-like) receptor 1, scavenger receptor class E, member 1

Reactivity

Rat

Detection Range

0.156 ng/mL-10 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human TF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-101
EDTA plasma (n=4)	98	93-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:50000	Average %	89
	Range %	84-95
1:100000	Average %	92
	Range %	87-97
1:200000	Average %	96
	Range %	90-101
1:400000	Average %	90
	Range %	86-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

nmol/L	OD1	OD2	Average	Corrected
1.5	2.367	2.396	2.382	2.278
0.75	1.898	1.753	1.826	1.722
0.375	1.262	1.288	1.275	1.171
0.187	0.948	0.982	0.965	0.861
0.094	0.583	0.597	0.590	0.486
0.047	0.353	0.348	0.351	0.247
0.023	0.284	0.295	0.290	0.186
0	0.102	0.106	0.104	?

Species

Rattus norvegicus

Sensitivity

0.039 ng/mL

Limit Of Detection

450 nm

UniProt ID	O70156
Antigen	oxidized low density lipoprotein (lectin-like) receptor 1

Applications

ELISA

Research Area

OLR1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508652	CSB-E15845r	96T	$595	Add to Cart
BHE10508652	CSB-E15845r	5x96T	$2856	Add to Cart
BHE10508652	CSB-E15845r	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.