Rat Vascular Endothelial Cell Growth Factor B

Mfr.No.

E0660Ra

Sensitivity

2.62ng/L

Detection Range

5-1000ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Vascular Endothelial Cell Growth Factor B (also known as VEGF-B) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Vascular endothelial growth factor B;VEGF B;VEGFB;VEGF-B;VEGF-related factor;VRF

Molecular Weight

21869

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E0660Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	192.8	9.65	5
Intra-Assay	2	18	358.8	12.39	3.5
Intra-Assay	3	18	109.8	6.98	6.4

Background

Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis (By similarity).

Protocol

These standard curves of E0660Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/L	2.175	2.112	2.059
	2.048
320ng/L	1.368	1.401	1.349
	1.435
160ng/L	0.723	0.789	0.736
	0.855
80ng/L	0.398	0.457	0.404
	0.516
40ng/L	0.299	0.291	0.238
	0.282
0ng/L	0.057	0.053	0
	0.048

Gene ID	89811
UniProt ID	O35485

Research Area	Cardiovascular; Signal Transduction; Neuroscience; Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111927	E0660Ra	48T	$320		Add to Cart
BHE12111927	E0660Ra	96T	$458		Add to Cart

Rat Vascular Endothelial Cell Growth Factor B - VEGF-B ELISA Kit

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