Sheep Corticoliberin - CRH ELISA Kit

Mfr.No.

E0162Sh

Sensitivity

1.54ng/L

Detection Range

3-900ng/L

Reactivity

Sheep

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Corticoliberin;Corticotropin-releasing factor;Corticotropin-releasing hormone;CRF;CRH;Endorpholiberin

Molecular Weight

20672

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E0162Sh within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	172.13	7.46	4.33
Intra-Assay	2	18	334.12	15.4	4.61
Intra-Assay	3	18	72.79	2.59	3.56

Background

Hormone regulating the release of corticotropin from pituitary gland (PubMed:6267699, PubMed:6273874). Induces NLRP6 in intestinal epithelial cells, hence may influence gut microbiota profile (By similarity).

Protocol

These standard curves of E0162Sh are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480ng/L	2.27	2.29	2.254
	2.31
240ng/L	1.393	1.379	1.344
	1.366
120ng/L	0.782	0.752	0.716
	0.721
60ng/L	0.292	0.37	0.334
	0.447
30ng/L	0.124	0.141	0.105
	0.158
0ng/L	0.026	0.036	0
	0.045

Gene ID	101121525
UniProt ID	P01142

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113630	E0162Sh	48T	$320		Add to Cart
BHE12113630	E0162Sh	96T	$458		Add to Cart

Sheep Corticoliberin - CRH ELISA Kit

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