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Home / Enzymes, Inhibitors & Substrates

RNase H, <i>E. coli</i>

SKU : BHZ10900013

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Applied Biological Materials (abm) Inc.

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Mfr.No.	E018
Description	RNase H (Ribonuclease H), E. coli is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA strands in RNA-DNA hybrids. This enzyme cleaves the 3'-O-P-bond of RNA and generates 3' hydroxyl and 5' phosphate products. RNase H does not digest single-stranded or double-stranded DNA and RNA.
Description	Removal of the poly(A) sequences from mRNA in the presence of oligo (dT) ; Directed cleavage of RNA ; Note: This product is not suitable for NGS or second strand cDNA synthesis applications.
Notes	One unit is defined as the amount of RNase H, E. coli that is required to hydrolyze 1 nmol of the RNA in radiolabeled poly(rA):poly(dT), to acid-soluble ribonucleotides in a total reaction volume of 50 μl in 20 minutes at 37°C in 1X RNase H, E. coli Reaction Buffer with 10 nmol radiolabeled poly(rA) and 12.5 μg poly(dT). This product is distributed for laboratory research only. Caution: Not for diagnostic use.
Concentration	5 U/μl
Form	Enzyme supplied with 10X Reaction Buffer
Quantity	500 U (100 μl)

Storage	Store all components at -20°C.
Shipping Condition	Dry Ice
Storage Condition	-20C
Buffer	Storage Buffer: 20 mM Tris-HCl (pH 7.5), 100 mM KCl, 10 mM MgCl2, 0.1 mM DTT, 0.1 mM EDTA, and 50% (v/v) Glycerol.
Format	Polymerase
Concentration	5 U/μl

Supporting Protocol	RNase H Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHZ10900013	E018	100µl	$63.25		Add to Cart

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