T5 Exonuclease(10 U/uL)

T5 Exonuclease is a double-stranded DNA-specific exonuclease and single-stranded DNA endonuclease. It can initiate digestion from the 5' end of single- or double-stranded DNA, as well as from gaps or nicks in linear or circular double-stranded DNA, but it does not degrade supercoiled double-stranded DNA. Therefore, T5 Exonuclease is widely used for removing incompletely ligated products from circularized dsDNA, degrading denatured DNA in alkaline extraction plasmids, degrading linear and nicked DNA in plasmid samples, and seamless cloning (Gibson assembly), etc.

Applications

In-Fusion Cloning

Degradation and alkaline lysis extraction of denatured DNA from plasmids

Specifications

Components

Shipping and Storage

The products should be stored at -25? ~ -15? for 3 years.

Figures

Figure1. Cloning Situation of Different Fragment Connections

Note: In-Fusion Cloning was carried out using T5 Exonuclease, with the connection of 2, 3, and 4 fragments respectively. The experimental results demonstrate that the application of T5 Exonuclease in In-Fusion Cloning can achieve a 100% positive clone rate (12 out of 12)

Documents:

Safety Data Sheet

14538_MSDS_Ver.EN20241210.pdf

Manuals

14538_Manual_Ver.EN20241210.pdf