JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Biochemicals / Small Molecules

Evans Blue tetrasodium salt

SKU : BHB21300128

An Antagonist of AMPA/Kainate receptors, P2X Receptors and a Potent Inhibitor of L-Glutamate Uptake

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

Alomone Labs

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	E-110, E-110_1 g, E-110_10 g, E-110_50 g
Description	Evans Blue tetrasodium salt (#E-110) is a highly pure, synthetic, and biologically active compound.
Molecular Formula	C34H24N6Na4O14S4.
Synonyms	Synthetic
Background	Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system and detailed knowledge of the properties of glutamate receptors is essential in understanding excitatory synaptic transmission in the brain. Glutamatergic transmission at excitatory synapses is mediated by three different classes of receptor-gated ion channels: AMPA receptors (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid), Kainate receptors and NMDA receptors (N-methyl-D-aspartate)1.The biphenyl derivative of 1,3-naphthalene disulfonic acid, known as Evans Blue tetrasodium salt, was found to reduce desensitization and partially block the peak amplitudes of non-NMDA-receptor mediated currents in rat thalamic neurons2, and to block kainate evoked currents of heterologously expressed recombinant AMPA receptors containing either subunit GluR1 or GluR2 (IC50 values are 220 uM)3. Evans Blue has been an important tool in many physiological and clinical investigations because of its high affinity for plasma albumin4.
Shipping	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Concentration	50 nM - 100 uM.
Reconstitution	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. For long-term storage of diluted solutions, we recommend adding 0.1% BSA. Repeat freeze-thawing may result in loss of activity.
Cas Number	314-13-6
Molecular Weight	960.8
Solubility	Water. Centrifuge all product preparations before use (10000 x g for 1 min).
Target	AMPA, Kainate, P2X receptors
Activity	Evans Blue tetrasodium salt blocks glutamate receptors with IC50s 150 nM and 220 nM for kainate-type GluR6 and AMPA-type GluR11.
Country Of Origin	Israel/IL
UNSPSC Code	41116134
Application	FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay
Lead Time	1-2 Business Days
Notes	Bioassay Tested: Yes Chemical Name: Tetrasodium;(6E)-4-amino-6-((4-(4-((2E)-2-(8-amino-1-oxo-5,7-disulfonatonaphthalen-2-ylidene)hydrazinyl)-3-methylphenyl)-2-methylphenyl)hydrazinylidene)-5-oxonaphthalene-1,3-disulfonate.

Purity	>90%
Form	Lyophilized powder.
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. Storage before Reconstitution: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. Storage of solutions: Up to four weeks at 4°C or three months at -20°C.

Product Manual	SDS

Reference	Seeburg, P. (1993) Trends Neurosci. 16, 359. Lessmann, V. et al. (1992) Neurosci. Lett. 146, 13. Keller, B.U. et al. (1993) Proc. Natl. Acad. Sci. U.S.A. 90, 605. Yen, L.F. et al. (2013) PLoS One 8, e68595. Price, C.J. and Raymond, L.A. (1996) Mol. Pharmacol. 50, 1665.

Have you used this product?

Rate it now..!!

Login to Review

Combination SKU	Supplier No.	Size	Your price	Quantity
BHB21300128-1	E-110_1 g	1 g	$37		Add to Cart
BHB21300128-2	E-110_10 g	10 g	$55		Add to Cart
BHB21300128-3	E-110_50 g	50 g	$206		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2025 Biohippo. All rights reserved.