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Home / Fish Probe

eFISH ROS1 Dual Color Break Apart P

SKU : BHR15100154

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Mfr.No.	FP060-10X
Gene Name	ROS1
Fluorophore	Orange/Green
Chromosome	6
Intended Use	The BioGenex eFISH ROS1 dual color break apart probe is currently available forResearch use only.eFISH ROS1 dual color break apart probe is designed to be used for thedetection of translocations involving the ROS1 gene at 6q22.1 in formalin-fixed,paraffinembedded tissue or cells by fluorescence in situ hybridization (FISH).BioGenex eFISH ROS1 dual color break apart probecomes in hybridization buffer. Theprobe contains green-labeled polynucleotides (Green: excitation at 503 nm and emissionat 528 nm, similar to FITC), which target sequences mapping in 6q22.1 proximal to theROS1 breakpoint cluster, and orangelabeled polynucleotides (Orange: excitation at 547nm and emission at 572 nm, similar to rhodamine), which target sequences mapping in6q22.1 distal to the ROS1 breakpoint cluster.
Description	Fluorescence in situ hybridization (FISH) is a robust technique of cytogenetic used for the detection of chromosomal aberrations, presence or absence of specific DNA sequence in native context. In this technique florescent probes bind to the target sequence of DNA in chromosome. High specificity and sensitivity coupled rapid and an accurate result has proven role of FISH in both research and diagnosis of solid tumor and hematological malignancies. As technique of cancer cytogenetics, FISH, can be used to identify genetic aberrations viz., deletions, amplification and translocation in tissue sections or within individual cells. FISH is also used for use in genetic counseling, medicine, and speciesidentification. FISH can also be used to detect and localize specific RNA targets in cells,circulating tumor cells, and tissue samples1,2,3,4,5.In FISH procedure, fixed tissue sections are pretreated to expose target DNA or mRNAsequences. An appropriately labeled probe is hybridized to the exposed target DNA ormRNA sequences in the cells. Subsequent stringent washing steps remove any probe thatis non-specifically bound to the tissue section. Subsequently slides are mounted usingDAPI/antifade and can be visualized under fluorescence microscope using appropriate filterset.
Principle	In Situ hybridization (ISH) allows the detection and localization of definitive nucleic acidsequences directly within a cell or tissue. High specificity is ensured through the action ofannealing of fluorescence probe nucleic acid sequence to complementary target nucleicacid sequence. ISH techniques can be used to identify genetic aberrations like deletions,amplification, and translocationin tissue sections or within individual cells.
Storage	The BioGenex eFISH ROS1 dual color break apart probe must be stored at 2-8°Cprotected from light and is stable through the expiry date printed on the label.

Reference	Datasheet SDS

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SKU	Supplier No.	Size	Your price	Quantity
BHR15100154	FP060-10X	10 tests	$1152		Add to Cart

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