Anti-TNF-alpha (Tumor Necrosis Factor alpha) Antibody BHA10205385

Mfr.No.	7124-MSM3-P0
Alternative Names	APC1 ; Cachectin ; Differentiation inducing factor (DIF) ; Macrophage cytotoxic factor (MCF) ; Necrosin ; TNF alpha ; TNF Macrophage Derived ; TNF Monocyte Derived ; TNF Superfamily Member 2 ; TNFA
Antigen	TNF-alpha (Tumor Necrosis Factor alpha)
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic, Extracellular(secreted)
Chromosome Location	6p21.33
Clonality	Monoclonal
Conjugate	Unconjugated
Description	This antibody neutralizes TNF alpha biological activities. It prevents TNF alpha induced apoptosis in Jurkat cells. It also neutralizes HurTNFamediated cytotoxicity of L929 cells and inhibits tumor growth in mice. It protects mice against toxicity of HurTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
Host	Mouse
Immunogen	Recombinant human TNF-alpha
Isotype	IgG1 Kappa
Positive Control	HeLa, HL-60, or A431 cells. Macrophages in lymph node or Tonsil
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	P01375
Uniprot	241570
Gene Id	7124

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA; 0.05% azide. Also available WITHOUT BSA; azide at 1.0mg/ml.
Concentration	200µg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab with BSA and Azide at 200μg/ml

Applications

FACS, IF

Description

Inhibits Tumor Growth (Order Ab without BSA/Azide); Neutralizes rTNF-alpha Mediated Cytotoxicity (Order Ab without BSA/Azide); Protects Mice Against Toxicity of rhTNF-alpha (Order Ab without BSA/Azide); Optimal dilution for a specific application should be determined., FACS ; IF

Dilution

FACS	0.5-1μg/million cells in 0.1ml
IF	1-2μg/ml

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SKU	Supplier No.	Size	Your price	Quantity
BHA10205385	7124-MSM3-P0	20µg	$204.67		Add to Cart
BHA10205385	7124-MSM3-P0	100µg	$393.20		Add to Cart

Anti-TNF-alpha (Tumor Necrosis Factor alpha) Antibody BHA10205385

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