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Home / Primary Antibodies / Flow Cytometry (FACS)

Anti-HL-60 Antigen / Human Leukemia Antigen Antibody BHA10207082

SKU : BHA10207082

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NeoBiotechnologies, Inc.

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Mfr.No.	MSM1-228-P0
Alternative Names	Not Known
Antigen	HL-60 Antigen / Human Leukemia Antigen
Categories	Primary Antibodies
Cellular Localization	Cell Surface
Clonality	Monoclonal
Conjugate	Unconjugated
Description	Reacts with human leukemia cell line HL-60 and immuno-precipitates two proteins with MW of 48kDa and 52kDa. It does not stain B-cell lines Daudi, PHS, Namalwa, RPMI-1788 and T-cell lines CCRF-HSB2, Jurkat and Molt-4. This antibody stains monocytes and up to 10% of lymphocytes from peripheral blood of healthy donors. Blast cells of patients with AMMonL (M5 following FAB classification), AMMonL (M4) and hairy cells leukemia are positive. Its antigen is particularly expressed on blood cells from patients with infectious mononucleosis and CLL. Histiocytes and macrophages are also positive. Malignant cells from patients with AML (M1 and M2), T-ALL, B-ALL are not stained.,HL-60 cells are used as an in vitro model of acute promyelocytic leukaemia and for differentiation and apoptosis studies. The HL60 cell line was established in 1977 from a patient with acute myeloid leukaemia. The cells largely resemble promyelocytes but can be induced to differentiate terminally in vitro. Some reagents cause HL60 cells to differentiate to granulocyte-like cells, others to monocyte/macrophage-like cells. The HL60 cell genome contains an amplified c-myc proto-oncogene; c-myc mRNA levels are correspondingly high in undifferentiated cells but decline rapidly following induction of differentiation.
Host	Mouse
Immunogen	Leukemia HL-60 cells
Positive Control	Monocytes in tonsil or spleen
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	Not Known
Uniprot	Not Known
Gene Id	Not Known

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA; 0.05% azide. Also available WITHOUT BSA; azide at 1.0mg/ml.
Concentration	200µg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab with BSA and Azide at 200μg/ml

Applications

FACS, IF

Description

Optimal dilution for a specific application should be determined., FACS ; IF

Dilution

FACS	0.5-1μg/million cells
IF	0.5-1μg/ml

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SKU	Supplier No.	Size	Your price	Quantity
BHA10207082	MSM1-228-P0	20µg	$204.67		Add to Cart
BHA10207082	MSM1-228-P0	100µg	$393.20		Add to Cart

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