Fluorescein-Labeled Mouse Anti-Human CD3 Monoclonal Antibody

Mfr.No.

A7592, A7593, A7991, 6610008, A8931, A7600, A7595, A7597

Description

The protein of CD3 together with the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The protein of CD3 plays an essential role in T-cell development. Defects in this gene cause immunodeficiency.

Isotype

mouse IgG1

Kit Components

The main component of the product is fluorescein-labeled mouse anti-human CD3 antibody, pH7.2 PBS Buffer, 1% ProClin300.

Specificity	Clone	Subtype	Fluorescence	Λ Ex	Λ Em
CD3	UCHT-1	IgG1	FITC	488nm	525nm
			PE	488nm	575nm
			PE-QB594	488nm	610nm
			PerCP	488nm	670nm
			PerCP-Cy5.5	488nm	695nm
			PE-Cy7	488nm	779nm
			APC	633nm	660nm
			APC-Cy7	633nm	779nm

Test Method

1. Take 2 tubes, add 100 μL of anticoagulated peripheral blood specimen, then add the recommended amount of the same fluorescein-labeled isotype control and this reagent respectively, and incubate at room temperature (18-25℃) for 15-30 minutes, protected from light.
2. Add 2mL of lysis separately, shake well and incubate for 10 minutes at room temperature (18-25℃) away from light.
3. Centrifuge at 300g for 5 minutes, discard the supernatant, add 2mL of PBS buffer to resuspend the cell precipitate, and shake well.
4. Centrifuge at 300g for 5 minutes, discard the supernatant, add 300μL PBS buffer to resuspend the cell precipitate, and incubate for 2 hours at 4°C, protected from light. If delayed detection (more than 2 hours) is required, use 300 μL of PBS buffer containing 1% paraformaldehyde to resuspend the cell precipitate and store at 2-8°C, protected from light, but the fixation time should not exceed 24 hours.

Note

1. If the flow cytometer is not calibrated, or if the fluorescence compensation is insufficient and the gate is incorrectly positioned, false results may be generated.
2. To obtain the optimum signal-to-noise ratio, the antibodies have been calibrated, so please use the reagents strictly in accordance with their recommended dosage.
3. The data measured with this product are not directly comparable with similar data obtained by other methodological reagents or instruments.

Expiration period: 18 months.
Production Date & Expiration Date: Refer to the packaging of the kit.

Clone No	UCHT1

Storage	Storage Conditions: 2~8℃, protected from light, DO NOT FREEZE.

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Combination SKU	Supplier No.	Size	Specification	Your price
BHA18400017-1	A7592	100T	FITC anti-human CD3	$80	Add to Cart
BHA18400017-2	A7593	100T	PE anti-human CD3	$100	Add to Cart
BHA18400017-3	A7991	100T	PE-QB610 anti-human CD3	$280	Add to Cart
BHA18400017-4	6610008	100T	PerCP anti-human CD3	$200	Add to Cart
BHA18400017-5	A8931	100T	PerCP-Cy5.5 anti-human CD3	$300	Add to Cart
BHA18400017-6	A7600	100T	PE-Cy7 anti-human CD3	$230	Add to Cart
BHA18400017-7	A7595	100T	APC anti-human CD3	$90	Add to Cart
BHA18400017-8	A7597	100T	APC-Cy7 anti-human CD3	$250	Add to Cart

Fluorescein-Labeled Mouse Anti-Human CD3 Monoclonal Antibody

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