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Home / Primary Antibodies

HIF1A Antibody / HIF-1 alpha (V2132SAF)

SKU : BHA17123472

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NSJ Bioreagents

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Mfr.No.	V2132SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (alpha and beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIFs regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	Recombinant human HIF1 alpha was used as the immunogen for this antibody.
Isotype	Mouse IgG2b, kappa
Species Reactivity	Human
Note	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HIF1 alpha antibody to be titered up or down for optimal performance.

Format	Purified
Purity	Protein G purified antibody
Storage	Store the HIF1 alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	FACS, IF
Description	Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17123472	V2132SAF-100UG	100 ug	$499		Add to Cart

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