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Home / Primary Antibodies / Polyclonal Antibodies

HSP70 (Fish) Antibody - SPC-314

BHA11907725
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Email
[email protected]
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Swissprot B5DG30
Accession Number NP_001135156.1
Gene Id 100196655
Research Area Cancer, Heat Shock, Cell Signaling, Chaperone Proteins, Tumor Biomarkers, Protein Trafficking
Purification Protein A Purified
Scientific Background HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (1). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (2). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (3). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (4). When cells are subjected to metabolic stress (e.g., heat shock) a member of the HSP 70 family, HSP 70 (HSP72), is expressed; HSP 70 is highly related to HSC70 (>90% sequence identity). Constitutively expressed HSC70 rapidly forms a stable complex with the highly inducible HSP70 in cells following heat shock. The interaction of HSC70 with HSP 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on HSC70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (5). Looking for more information on HSP70 Visit our new HSP70 Scientific Resource Guide at http://www.HSP70.com.
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~70kDa. It does not cross react with HSC70. It does not detect HSP70 in other species.
Storage Buffer Lyophilized rabbit Antiserum. For reconstitution add 200 ul of sterile water.
Storage Temperature -20°C
Applications WB, IHC, IP
Dilution WB (1:1000), IHC (1:100), IP (5ug); optimal dilutions for assays should be determined by the user.
Product Manual SDS
Reference 1. Boorstein W.R., Ziegelhoffer T., and Craig E.A. (1993) J. Mol. Evol. 38(1): 1-17.

2. Rothman J. (1989) Cell. 59: 591-601.

3. DeLuca-Flaherty et al. (1990) Cell. 62: 875-887.

4. Bork P., Sander C., and Valencia A. (1992) Proc. Natl Acad. Sci. USA. 89: 7290-7294.

5. Brown C.L. et al. (1996) J. Biol. Chem. 271(2): 833-840.
Combination SKU Supplier No. Size Conjugate Your price Quantity
BHA11907725-1 SPC-314B 200 ug Unconjugated $403
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