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Home / ELISA Kit

Human Carboxyterminal propeptide of type I procollagen (PICP) ELISA Kit

SKU : BHE13703546

The carboxyterminal propeptide of type I procollagen (PICP) and the aminoterminal propeptide of type I procollagen (PINP) are enzymatically removed from the procollagen molecule by specific proteases.
PICP and PINP are indicators of the synthesis of type I collagen. Cleavage of PICP is required for the initiation of fibril formation, whereas PINP may be retained in the collagen molecule resulting in type I pN-collagen. The retention of PINP is transient, unlike the presence of IIIpN-collagen, and has been considered to regulate fibril diameter (Fleischmajer et al. 1985).
PICP is a trimeric, globular protein consisting of three polypeptide chains: two proalpha1(I) and one proalpha2(I) chains. The molecular mass of PICP is 100 000 and it is cleared by mannose receptors in liver endothelial cells (Smedsrød et al. 1990).

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Mfr.No.	KTE61330
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Carboxyterminal propeptide of type I procollagen (PICP) ELISA Kit employs a two-site sandwich ELISA to quantitate PINP.
Alternative Names	PINP
Detection Method	Colorimetric
Kit Components	Human Carboxyterminal propeptide of type I procollagen microplate Human Carboxyterminal propeptide of type I procollagen standard Human Carboxyterminal propeptide of type I procollagen detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Carboxyterminal propeptide of type I procollagen (PICP) ELISA Kit has high sensitivity and excellent specificity for detection of Human PINP. No significant cross-reactivity or interference between Human PINP and analogues was observed.
Calibration Range	Please inquire
Sample Type	Serum, Cell culture supernatants, Plasma, Other biological fluids
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The carboxyterminal propeptide of type I procollagen (PICP) and the aminoterminal propeptide of type I procollagen (PINP) are enzymatically removed from the procollagen molecule by specific proteases. PICP and PINP are indicators of the synthesis of type I collagen. Cleavage of PICP is required for the initiation of fibril formation, whereas PINP may be retained in the collagen molecule resulting in type I pN-collagen. The retention of PINP is transient, unlike the presence of IIIpN-collagen, and has been considered to regulate fibril diameter (Fleischmajer et al. 1985). PICP is a trimeric, globular protein consisting of three polypeptide chains: two proalpha1(I) and one proalpha2(I) chains. The molecular mass of PICP is 100 000 and it is cleared by mannose receptors in liver endothelial cells (Smedsrød et al. 1990).
Notes	This Human Carboxyterminal propeptide of type I procollagen (PICP) ELISA Kit employs a two-site sandwich ELISA to quantitate PINP in samples. An antibody specific for PINP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPINP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PINP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PINP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Carboxyterminal propeptide of type I procollagen (PICP) ELISA Kit employs a two-site sandwich ELISA to quantitate PINP in samples. An antibody specific for PINP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPINP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PINP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PINP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703546	KTE61330	48 T	$359		Add to Cart
BHE13703546	KTE61330	96 T	$619		Add to Cart
BHE13703546	KTE61330	96 Tx5	$2809		Add to Cart
BHE13703546	KTE61330	96 Tx50	$26209		Add to Cart

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