Matrices listed below were spiked with certain level of CEMIP and the recovery rates were calculated by comparing the measured value to the expected amount of CEMIP in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-98
93
EDTA plasma(n=5)
91-105
99
heparin plasma(n=5)
87-101
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CEMIP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-104%
96-103%
91-104%
EDTA plasma(n=5)
88-95%
82-100%
88-100%
heparin plasma(n=5)
82-96%
81-94%
86-100%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q8WUJ3
Antigen
CEMIP
Applications
ELISA
Applications Notes
CEMIP ELISA Kit allows for the in vitro quantitative determination of CEMIP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human CEMIP(Cell migRation-inducing and hyaluronan-binding protein) ELISA Kit