Matrices listed below were spiked with certain level of CHAT and the recovery rates were calculated by comparing the measured value to the expected amount of CHAT in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
96
EDTA plasma(n=5)
87-105
95
heparin plasma(n=5)
91-103
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CHAT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
CHAT ELISA Kit allows for the in vitro quantitative determination of CHAT concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human CHAT(Choline O-acetyltransferase) ELISA Kit