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Home / ELISA Kit

Human DNA replication licensing factor MCM3 (MCM3) ELISA Kit

SKU : BHE13703906

MCM3 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins.
This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.

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Mfr.No.	KTE61691
Description	This Human DNA replication licensing factor MCM3 (MCM3) ELISA Kit employs a two-site sandwich ELISA to quantitate MCM3.
Alternative Names	MCM3; HCC5; MGC1157; P1-MCM3; P1.h; RLFB; DNA polymerase alpha holoenzyme-associated protein P1; DNA replication factor MCM3; MCM3 minichromosome maintenance deficient 3; cervical cancer proto-oncogene 5; hRl
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human DNA replication licensing factor MCM3 microplate Human DNA replication licensing factor MCM3 standard Human DNA replication licensing factor MCM3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human DNA replication licensing factor MCM3 (MCM3) ELISA Kit has high sensitivity and excellent specificity for detection of Human MCM3. No significant cross-reactivity or interference between Human MCM3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MCM3 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.
Notes	This Human DNA replication licensing factor MCM3 (MCM3) ELISA Kit employs a two-site sandwich ELISA to quantitate MCM3 in samples. An antibody specific for MCM3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMCM3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCM3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCM3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	4172
Antigen	MCM3
Tag	MCM3
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human DNA replication licensing factor MCM3 (MCM3) ELISA Kit employs a two-site sandwich ELISA to quantitate MCM3 in samples. An antibody specific for MCM3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMCM3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCM3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCM3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703906	KTE61691	48 T	$339		Add to Cart
BHE13703906	KTE61691	96 T	$589		Add to Cart
BHE13703906	KTE61691	96 Tx5	$2629		Add to Cart
BHE13703906	KTE61691	96 Tx50	$24569		Add to Cart

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