Human epidermal growth factor receptor,EGFR ELISA Kit

Mfr.No.

CSB-E12124h

Alternative Names

epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian), ERBB, ERBB1, HER1, PIG61, mENA, avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, epidermal growth factor recept

Reactivity

Human

Detection Range

0.312 ng/mL-20 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of pig HAVCR1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	86-100
EDTA plasma (n=4)	96	90-110

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig HAVCR1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-101
1:2	Average %	93
	Range %	82-102
1:4	Average %	95
	Range %	88-99
1:8	Average %	95
	Range %	90-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
12	1.786	1.793	1.790	1.653
6	1.405	1.456	1.431	1.294
3	1.034	1.008	1.021	0.884
1.5	0.669	0.682	0.676	0.539
0.75	0.435	0.448	0.442	0.305
0.375	0.288	0.303	0.296	0.159
0.18	0.185	0.191	0.188	0.051
0	0.135	0.138	0.137	?

Species

Homo sapiens

Sensitivity

0.019 ng/mL

Limit Of Detection

450 nm

UniProt ID	P00533
Antigen	epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)

Applications

ELISA

Research Area

EGFR

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502277	CSB-E12124h	96T	$445	Add to Cart
BHE10502277	CSB-E12124h	5x96T	$2136	Add to Cart
BHE10502277	CSB-E12124h	10x96T	$4227.50	Add to Cart

Human epidermal growth factor receptor,EGFR ELISA Kit

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