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Home / ELISA Kit

Human High mobility group box 1 protein (HMGB1) ELISA Kit

SKU : BHE13704943

employs a two-site sandwich ELISA to quantitate HMGB1 in samples.

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Abbkine Scientific Co., Ltd.

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Mfr.No.	KTE62814
Description	employs a two-site sandwich ELISA to quantitate HMGB1 in samples.
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich
Calibration Range	10 ug/L-160 ug/L
Limit Of Detection	1.0 ug/L
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Notes	Human High mobility group box 1 protein (HMGB1) ELISA Kit employs a two-site sandwich ELISA to quantitate HMGB1 in samples. An antibody specific for HMGB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HMGB1 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human HMGB1 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of HMGB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Antigen	HMGB1
Tag	HMGB1
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	Human High mobility group box 1 protein (HMGB1) ELISA Kit employs a two-site sandwich ELISA to quantitate HMGB1 in samples. An antibody specific for HMGB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HMGB1 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human HMGB1 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of HMGB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13704943	KTE62814	48 T	$359		Add to Cart
BHE13704943	KTE62814	96 T	$619		Add to Cart
BHE13704943	KTE62814	96 Tx5	$2809		Add to Cart
BHE13704943	KTE62814	96 Tx50	$26209		Add to Cart

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