Human IL1q(Interleukin 1 Theta) ELISA Kit

Mfr.No.

ELK7424

Alternative Names

IL1F10; IL38; FKSG75; IL-1HY2; IL-1F10; FIL1-Theta; Interleukin-38; Interleukin-1 Receptor Antagonist FKSG75; Interleukin 1 Family, Member 10

Reactivity

Human

Detection Range

7.82-500 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Other biological fluids, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant IL1q and the recovery rates were calculated by comparing the measured value to the expected amount of IL1q in samples.

Matrix	Recovery range	Average
serum(n=5)	93-101%	97%
EDTA plasma(n=5)	85-103%	94%
Heparin plasma(n=5)	95-105%	100%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL1q and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	86-93%	91-103%	87-98%	92-99%
EDTA plasma(n=5)	89-99%	83-103%	98-105%	96-101%
Heparin plasma(n=5)	85-105%	84-98%	95-102%	88-102%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
500.00	1.861	1.773
250.00	1.700	1.612
125.00	1.195	1.107
62.50	0.871	0.783
31.25	0.539	0.451
15.63	0.387	0.299
7.82	0.214	0.126
0.00	0.088	0.000

Sensitivity

2.7 pg/mL

Limit Of Detection

500 pg/mL

Antigen	IL1q
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL1q. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL1q. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL1q, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL1q in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Signal transduction;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15201070	ELK7424	48T	$320		Add to Cart
BHE15201070	ELK7424	96T	$458		Add to Cart

Human IL1q(Interleukin 1 Theta) ELISA Kit

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