Human Interferon Regulatory Factor 4

Mfr.No.

E3334Hu

Description

This sandwich kit is for the accurate quantitative detection of human Interferon Regulatory Factor 4 (also known as IRF4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Interferon regulatory factor 4;IRF4;IRF-4;LSIRF;Lymphocyte-specific interferon regulatory factor;Multiple myeloma oncogene 1;MUM1;NF-EM5;SHEP8;

Reactivity

Human

Detection Range

5-1500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

51772

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

2.58ng/L

Background

Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes (By similarity).

Gene ID	3662
UniProt ID	Q15306
Antigen	IRF4

Research Area

Tags & Cell Markers; Epigenetics and Nuclear Signaling; Cancer

Protocol

These standard curves of E3334Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.106	2.095	2.06
	2.084
600ng/L	1.506	1.539	1.505
	1.572
300ng/L	0.603	0.603	0.569
	0.604
150ng/L	0.396	0.419	0.385
	0.442
75ng/L	0.172	0.171	0.136
	0.17
0ng/L	0.026	0.034	0
	0.042

Precision

We measured random samples of E3334Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	326.33	10.86	3.33
Intra-Assay	2	18	528.39	14.8	2.8
Intra-Assay	3	18	245.97	9.66	3.93

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104863	E3334Hu	48T	$320		Add to Cart
BHE12104863	E3334Hu	96T	$458		Add to Cart

Human Interferon Regulatory Factor 4 - IRF4 ELISA Kit

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