Human Interleukin-1 Family Member 10

Mfr.No.

E4686Hu

Description

This sandwich kit is for the accurate quantitative detection of human Interleukin-1 Family Member 10 (also known as IL1F10) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Family of interleukin 1-theta;FIL1 theta;IL 1F10;IL-1 theta;IL1F10;IL-1F10;IL-1HY2;IL-38;Interleukin-1 family member 10;Interleukin-1 HY2;Interleukin-1 theta;Interleukin-38

Reactivity

Human

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

16943

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

11.15ng/L

Background

Cytokine with immunomodulatory activity. Alone, does not induce cytokine production, but reduces IL22 and IL17A production by T-cells in response to heat-killed Candida albicans. Reduces IL36G-induced production of IL8 by peripheral blood mononuclear cells. Increases IL6 production by dendritic cells stimulated by bacterial lipopolysaccharides (LPS). Ligand for IL-36R/IL1RL2.

Gene ID	84639
UniProt ID	Q8WWZ1
Antigen	IL1F10

Research Area

Immunology

Protocol

These standard curves of E4686Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.365	2.265	2.233
	2.164
1200ng/L	1.343	1.287	1.256
	1.231
600ng/L	0.719	0.729	0.697
	0.738
300ng/L	0.291	0.36	0.329
	0.43
150ng/L	0.133	0.141	0.109
	0.148
0ng/L	0.042	0.031	0
	0.021

Precision

We measured random samples of E4686Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	540	32.73	6.1
Intra-Assay	2	18	883.7	44.72	5.1
Intra-Assay	3	18	1300.6	35.75	2.7

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105980	E4686Hu	48T	$320		Add to Cart
BHE12105980	E4686Hu	96T	$458		Add to Cart

Human Interleukin-1 Family Member 10 - IL1F10 ELISA Kit

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