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Home / ELISA Kit

Human Leukotriene E4 (LT-E4) ELISA Kit

SKU : BHE13703982

A biologically active principle of SRS-A that is formed from LEUKOTRIENE D4 via a peptidase reaction that removes the glycine residue. The biological actions of LTE4 are similar to LTC4 and LTD4.

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Abbkine Scientific Co., Ltd.

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Mfr.No.	KTE61767
Description	This Human Leukotriene E4 (LT-E4) ELISA Kit employs a two-site sandwich ELISA to quantitate LT-E4.
Alternative Names	LT-E4
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Leukotriene E4 microplate Human Leukotriene E4 standard Human Leukotriene E4 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Leukotriene E4 (LT-E4) ELISA Kit has high sensitivity and excellent specificity for detection of Human LT-E4. No significant cross-reactivity or interference between Human LT-E4 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	A biologically active principle of SRS-A that is formed from LEUKOTRIENE D4 via a peptidase reaction that removes the glycine residue. The biological actions of LTE4 are similar to LTC4 and LTD4.
Notes	This Human Leukotriene E4 (LT-E4) ELISA Kit employs a two-site sandwich ELISA to quantitate LT-E4 in samples. An antibody specific for LT-E4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLT-E4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LT-E4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LT-E4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Antigen	LT-E4
Tag	LT-E4
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Leukotriene E4 (LT-E4) ELISA Kit employs a two-site sandwich ELISA to quantitate LT-E4 in samples. An antibody specific for LT-E4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLT-E4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LT-E4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LT-E4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703982	KTE61767	48 T	$259		Add to Cart
BHE13703982	KTE61767	96 T	$469		Add to Cart
BHE13703982	KTE61767	96 Tx5	$2109		Add to Cart
BHE13703982	KTE61767	96 Tx50	$18719		Add to Cart

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