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Home / ELISA Kit

Human Low-density lipoprotein-receptor-related protein (LRP-6) ELISA Kit

SKU : BHE13703996

The LRP6 is a member of the low density lipoprotein (LDL) receptor gene family. LDL receptors are transmembrane cell surface proteins involved in receptor-mediated endocytosis of lipoprotein and protein ligands. The protein encoded by this gene functions as a receptor or, with Frizzled, a co-receptor for Wnt and thereby transmits the canonical Wnt/beta-catenin signaling cascade. Through its interaction with the Wnt/beta-catenin signaling cascade this gene plays a role in the regulation of cell differentiation, proliferation, and migration and the development of many cancer types. This protein undergoes gamma-secretase dependent RIP- (regulated intramembrane proteolysis) processing but the precise locations of the cleavage sites have not been determined.

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Mfr.No.	KTE61781
Description	This Human Low-density lipoprotein-receptor-related protein (LRP-6) ELISA Kit employs a two-site sandwich ELISA to quantitate LRP6.
Alternative Names	LRP6; ADCAD2; FLJ90062; FLJ90421;
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Low-density lipoprotein-receptor-related protein microplate Human Low-density lipoprotein-receptor-related protein standard Human Low-density lipoprotein-receptor-related protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Low-density lipoprotein-receptor-related protein (LRP-6) ELISA Kit has high sensitivity and excellent specificity for detection of Human LRP6. No significant cross-reactivity or interference between Human LRP6 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The LRP6 is a member of the low density lipoprotein (LDL) receptor gene family. LDL receptors are transmembrane cell surface proteins involved in receptor-mediated endocytosis of lipoprotein and protein ligands. The protein encoded by this gene functions as a receptor or, with Frizzled, a co-receptor for Wnt and thereby transmits the canonical Wnt/beta-catenin signaling cascade. Through its interaction with the Wnt/beta-catenin signaling cascade this gene plays a role in the regulation of cell differentiation, proliferation, and migration and the development of many cancer types. This protein undergoes gamma-secretase dependent RIP- (regulated intramembrane proteolysis) processing but the precise locations of the cleavage sites have not been determined.
Notes	This Human Low-density lipoprotein-receptor-related protein (LRP-6) ELISA Kit employs a two-site sandwich ELISA to quantitate LRP6 in samples. An antibody specific for LRP6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLRP6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LRP6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LRP6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	4040
Antigen	LRP6
Tag	LRP6
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Low-density lipoprotein-receptor-related protein (LRP-6) ELISA Kit employs a two-site sandwich ELISA to quantitate LRP6 in samples. An antibody specific for LRP6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLRP6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LRP6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LRP6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703996	KTE61781	48 T	$359		Add to Cart
BHE13703996	KTE61781	96 T	$619		Add to Cart
BHE13703996	KTE61781	96 Tx5	$2809		Add to Cart
BHE13703996	KTE61781	96 Tx50	$26209		Add to Cart

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