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Home / ELISA Kit

Human Mesoderm development candidate 1 (MESDC1) ELISA Kit

SKU : BHE13703864

MESDC1 encodes Mesoderm development candidate 1 that is regulated by micro RNA MiR-574-3, and is thought to have an oncogenic function in human bladder cancer. A similar gene in mouse is located in a chromosomal region critical for differentiation of mesoderm, which affects embryo patterning and the formation of heart, muscle, blood, skeleton and the urogenital system. The mouse gene is expressed in early development, and in the adult.

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Mfr.No.	KTE61649
Description	This Human Mesoderm development candidate 1 (MESDC1) ELISA Kit employs a two-site sandwich ELISA to quantitate MESDC1.
Alternative Names	MESDC1; MGC99595;
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Mesoderm development candidate 1 microplate Human Mesoderm development candidate 1 standard Human Mesoderm development candidate 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Mesoderm development candidate 1 (MESDC1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MESDC1. No significant cross-reactivity or interference between Human MESDC1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MESDC1 encodes Mesoderm development candidate 1 that is regulated by micro RNA MiR-574-3, and is thought to have an oncogenic function in human bladder cancer. A similar gene in mouse is located in a chromosomal region critical for differentiation of mesoderm, which affects embryo patterning and the formation of heart, muscle, blood, skeleton and the urogenital system. The mouse gene is expressed in early development, and in the adult.
Notes	This Human Mesoderm development candidate 1 (MESDC1) ELISA Kit employs a two-site sandwich ELISA to quantitate MESDC1 in samples. An antibody specific for MESDC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMESDC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MESDC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MESDC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	59274
Antigen	MESDC1
Tag	MESDC1
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Mesoderm development candidate 1 (MESDC1) ELISA Kit employs a two-site sandwich ELISA to quantitate MESDC1 in samples. An antibody specific for MESDC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMESDC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MESDC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MESDC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703864	KTE61649	48 T	$339		Add to Cart
BHE13703864	KTE61649	96 T	$589		Add to Cart
BHE13703864	KTE61649	96 Tx5	$2629		Add to Cart
BHE13703864	KTE61649	96 Tx50	$24569		Add to Cart

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