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Home / ELISA Kit

Human Methyl-CpG-binding domain protein 1 (MBD1) ELISA Kit

SKU : BHE13703919

DPEP1 (EC 3.4.13.11) is a kidney membrane enzyme that hydrolyzes a variety of dipeptides and is implicated in renal metabolism of glutathione and its conjugates, e.g., leukotriene D4 (Kozak and Tate, 1982).DPEP1 is responsible for hydrolysis of the beta-lactam ring of antibiotics, such as penem and carbapenem (Campbell et al., 1984). Earlier, beta-lactamase enzymes were thought to occur only in bacteria, where their probable function was in protecting the organisms against the action of beta-lactam antibiotics. These antibiotics exhibit selective toxicity against bacteria but virtual inertness against many eukaryotic cells (Adachi et al., 1990).

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Mfr.No.	KTE61704
Description	This Human Methyl-CpG-binding domain protein 1 (MBD1) ELISA Kit employs a two-site sandwich ELISA to quantitate MBD1.
Alternative Names	MBD1; CXXC3; PCM1; RFT; OTTHUMP00000163504; OTTHUMP00000163506; OTTHUMP00000163507; methyl-CpG binding domain protein 1 isoform PCM1; the regulator of fibroblast growth factor 2 (FGF-2) transcription
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Methyl-CpG-binding domain protein 1 microplate Human Methyl-CpG-binding domain protein 1 standard Human Methyl-CpG-binding domain protein 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Methyl-CpG-binding domain protein 1 (MBD1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MBD1. No significant cross-reactivity or interference between Human MBD1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	DPEP1 (EC 3.4.13.11) is a kidney membrane enzyme that hydrolyzes a variety of dipeptides and is implicated in renal metabolism of glutathione and its conjugates, e.g., leukotriene D4 (Kozak and Tate, 1982).DPEP1 is responsible for hydrolysis of the beta-lactam ring of antibiotics, such as penem and carbapenem (Campbell et al., 1984). Earlier, beta-lactamase enzymes were thought to occur only in bacteria, where their probable function was in protecting the organisms against the action of beta-lactam antibiotics. These antibiotics exhibit selective toxicity against bacteria but virtual inertness against many eukaryotic cells (Adachi et al., 1990).
Notes	This Human Methyl-CpG-binding domain protein 1 (MBD1) ELISA Kit employs a two-site sandwich ELISA to quantitate MBD1 in samples. An antibody specific for MBD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMBD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MBD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MBD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	4152
Antigen	MBD1
Tag	MBD1
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Methyl-CpG-binding domain protein 1 (MBD1) ELISA Kit employs a two-site sandwich ELISA to quantitate MBD1 in samples. An antibody specific for MBD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMBD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MBD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MBD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703919	KTE61704	48 T	$339		Add to Cart
BHE13703919	KTE61704	96 T	$589		Add to Cart
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