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Home / ELISA Kit

Human Microtubule-associated tumor suppressor 1 (MTUS1) ELISA Kit

SKU : BHE13703685

MTUS1 encodes a protein which contains a C-terminal domain able to interact with the angiotension II (AT2) receptor and a large coiled-coil region allowing dimerization. Multiple alternatively spliced transcript variants encoding different isoforms have been found for MTUS1. One of the transcript variants has been shown to encode a mitochondrial protein that acts as a tumor suppressor and partcipates in AT2 signaling pathways. Other variants may encode nuclear or transmembrane proteins but it has not been determined whether they also participate in AT2 signaling pathways.

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Mfr.No.	KTE61470
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Microtubule-associated tumor suppressor 1 (MTUS1) ELISA Kit employs a two-site sandwich ELISA to quantitate MTUS1.
Alternative Names	MTUS1; ATBP; ATIP; DKFZp586D1519; DKFZp686F20243; FLJ14295; KIAA1288; MP44; MTSG1; AT2 receptor-interacting protein; AT2R binding protein; erythroid differentiation-related; mitochondrial tumor suppressor 1; m
Detection Method	Colorimetric
Kit Components	Human Microtubule-associated tumor suppressor 1 microplate Human Microtubule-associated tumor suppressor 1 standard Human Microtubule-associated tumor suppressor 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Microtubule-associated tumor suppressor 1 (MTUS1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MTUS1. No significant cross-reactivity or interference between Human MTUS1 and analogues was observed.
Calibration Range	Please inquire
Sample Type	Serum, Cell culture supernatants, Plasma, Other biological fluids
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MTUS1 encodes a protein which contains a C-terminal domain able to interact with the angiotension II (AT2) receptor and a large coiled-coil region allowing dimerization. Multiple alternatively spliced transcript variants encoding different isoforms have been found for MTUS1. One of the transcript variants has been shown to encode a mitochondrial protein that acts as a tumor suppressor and partcipates in AT2 signaling pathways. Other variants may encode nuclear or transmembrane proteins but it has not been determined whether they also participate in AT2 signaling pathways.
Notes	This Human Microtubule-associated tumor suppressor 1 (MTUS1) ELISA Kit employs a two-site sandwich ELISA to quantitate MTUS1 in samples. An antibody specific for MTUS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTUS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTUS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTUS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	57509

Applications	ELISA
Applications Notes	This Human Microtubule-associated tumor suppressor 1 (MTUS1) ELISA Kit employs a two-site sandwich ELISA to quantitate MTUS1 in samples. An antibody specific for MTUS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTUS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTUS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTUS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703685	KTE61470	48 T	$339		Add to Cart
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