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Home / ELISA Kit

Human Myogenic factor 5 (MYF5) ELISA Kit

SKU : BHE13703663

MYF5 (Myogenic Factor 5) is a Protein Coding gene. Diseases associated with MYF5 include Rhabdomyosarcoma and Skeletal Muscle Cancer. Among its related pathways are Transcriptional Regulatory Network in Embryonic Stem Cell and CDO in myogenesis. GO annotations related to this gene include sequence-specific DNA binding and RNA polymerase II core promoter proximal region sequence-specific DNA binding. An important paralog of this gene is MYOD1. Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYOG and MYOD1, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.

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Mfr.No.	KTE61448
Description	This Human Myogenic factor 5 (MYF5) ELISA Kit employs a two-site sandwich ELISA to quantitate MYF5.
Alternative Names	MYF5; bHLHc2;
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Myogenic factor 5 microplate Human Myogenic factor 5 standard Human Myogenic factor 5 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Myogenic factor 5 (MYF5) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYF5. No significant cross-reactivity or interference between Human MYF5 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MYF5 (Myogenic Factor 5) is a Protein Coding gene. Diseases associated with MYF5 include Rhabdomyosarcoma and Skeletal Muscle Cancer. Among its related pathways are Transcriptional Regulatory Network in Embryonic Stem Cell and CDO in myogenesis. GO annotations related to this gene include sequence-specific DNA binding and RNA polymerase II core promoter proximal region sequence-specific DNA binding. An important paralog of this gene is MYOD1. Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYOG and MYOD1, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
Notes	This Human Myogenic factor 5 (MYF5) ELISA Kit employs a two-site sandwich ELISA to quantitate MYF5 in samples. An antibody specific for MYF5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYF5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYF5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYF5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	4617
Antigen	MYF5
Tag	MYF5
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Myogenic factor 5 (MYF5) ELISA Kit employs a two-site sandwich ELISA to quantitate MYF5 in samples. An antibody specific for MYF5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYF5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYF5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYF5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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