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Home / ELISA Kit

Human Prostatic Acid Phosphatase (PAP) ELISA Kit

SKU : BHE13703522

Prostatic acid phosphatase (PAP) is an enzyme produced by the prostate. It may be found in increased amounts in men who have prostate cancer or other diseases.The highest levels of acid phosphatase are found in metastasized prostate cancer. Diseases of the bone, such as Paget's disease or hyperparathyroidism, diseases of blood cells, such as sickle-cell disease or multiple myeloma or lysosomal storage diseases, such as Gaucher's disease, will show moderately increased levels.Certain medications can cause temporary increases or decreases in acid phosphatase levels. Manipulation of the prostate gland through massage, biopsy or rectal exam before a test may increase the level.Its physiological function may be associated with the liquefaction process of semen.

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Mfr.No.	KTE61306
Description	This Human Prostatic Acid Phosphatase (PAP) ELISA Kit employs a two-site sandwich ELISA to quantitate PAP.
Alternative Names	PAP
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Prostatic Acid Phosphatase microplate Human Prostatic Acid Phosphatase standard Human Prostatic Acid Phosphatase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Prostatic Acid Phosphatase (PAP) ELISA Kit has high sensitivity and excellent specificity for detection of Human PAP. No significant cross-reactivity or interference between Human PAP and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Prostatic acid phosphatase (PAP) is an enzyme produced by the prostate. It may be found in increased amounts in men who have prostate cancer or other diseases.The highest levels of acid phosphatase are found in metastasized prostate cancer. Diseases of the bone, such as Paget's disease or hyperparathyroidism, diseases of blood cells, such as sickle-cell disease or multiple myeloma or lysosomal storage diseases, such as Gaucher's disease, will show moderately increased levels.Certain medications can cause temporary increases or decreases in acid phosphatase levels. Manipulation of the prostate gland through massage, biopsy or rectal exam before a test may increase the level.Its physiological function may be associated with the liquefaction process of semen.
Notes	This Human Prostatic Acid Phosphatase (PAP) ELISA Kit employs a two-site sandwich ELISA to quantitate PAP in samples. An antibody specific for PAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	55
Antigen	PAP
Tag	PAP
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Prostatic Acid Phosphatase (PAP) ELISA Kit employs a two-site sandwich ELISA to quantitate PAP in samples. An antibody specific for PAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703522	KTE61306	48 T	$359		Add to Cart
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BHE13703522	KTE61306	96 Tx50	$26209		Add to Cart

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