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Home / ELISA Kit

Human Protein disulfide isomerase (PDI) ELISA Kit

SKU : BHE13703541

PROHB is the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

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Mfr.No.	KTE61325
Description	This Human Protein disulfide isomerase (PDI) ELISA Kit employs a two-site sandwich ELISA to quantitate P4HB.
Alternative Names	P4HB; DSI; ERBA2L; GIT; P4Hbeta; PDI; PDIA1; PHDB; PO4DB; PO4HB; PROHB; collagen prolyl 4-hydroxylase beta; glutathione-insulin transhydrogenase; procollagen-proline; 2-oxoglutarate 4-dioxygenase (proline 4
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Protein disulfide isomerase microplate Human Protein disulfide isomerase standard Human Protein disulfide isomerase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Protein disulfide isomerase (PDI) ELISA Kit has high sensitivity and excellent specificity for detection of Human P4HB. No significant cross-reactivity or interference between Human P4HB and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	PROHB is the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.
Notes	This Human Protein disulfide isomerase (PDI) ELISA Kit employs a two-site sandwich ELISA to quantitate P4HB in samples. An antibody specific for P4HB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyP4HB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for P4HB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of P4HB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	5034
Antigen	P4HB
Tag	P4HB
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Protein disulfide isomerase (PDI) ELISA Kit employs a two-site sandwich ELISA to quantitate P4HB in samples. An antibody specific for P4HB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyP4HB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for P4HB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of P4HB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703541	KTE61325	48 T	$359		Add to Cart
BHE13703541	KTE61325	96 T	$619		Add to Cart
BHE13703541	KTE61325	96 Tx5	$2809		Add to Cart
BHE13703541	KTE61325	96 Tx50	$26209		Add to Cart

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