Human PYCARD(Apoptosis-associated speck-like protein containing a CARD) ELISA Kit

Mfr.No.

EH15191

Description

Human PYCARD(Apoptosis-associated speck-like protein containing a CARD) ELISA Kit

Alternative Names

hASC ELISA Kit, Caspase recruitment domain-containing protein 5 ELISA Kit, PYD and CARD domain-containing protein ELISA Kit, Target of methylation-induced silencing 1 ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

78.125-5000pg/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of PYCARD and the recovery rates were calculated by comparing the measured value to the expected amount of PYCARD in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	93-105	99
EDTA plasma(n=5)	89-101	95
heparin plasma(n=5)	88-104	97

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PYCARD and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	93-98%	86-105%	87-103%
EDTA plasma(n=5)	84-100%	88-98%	86-96%
heparin plasma(n=5)	85-87%	81-98%	80-91%

Sensitivity

< 46.875pg/ml

Notes

For Research Use Only

UniProt ID	Q9ULZ3

Applications	ELISA
Applications Notes	PYCARD ELISA Kit allows for the in vitro quantitative determination of PYCARD concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10807530	EH15191	96T	$400		Add to Cart

Human PYCARD(Apoptosis-associated speck-like protein containing a CARD) ELISA Kit

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