Human TNFSF11/RANKL(Tumor necrosis factor ligand superfamily member 11) ELISA Kit

Mfr.No.

EH0313

Alternative Names

RANKL ELISA Kit, TNFSF11 ELISA Kit, CD254 ELISA Kit, ODF ELISA Kit, OPGL ELISA Kit, RANK L ELISA Kit, TNFSF11 ELISA Kit, CD254 antigen ELISA Kit, OPGLOPTB2 ELISA Kit, TRANCEODFhRANKL2 ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

78.125-5000pg/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of TNFSF11/RANKL and the recovery rates were calculated by comparing the measured value to the expected amount of TNFSF11/RANKL in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	86-101	92
EDTA plasma(n=5)	87-104	97
heparin plasma(n=5)	97-103	100

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFSF11/RANKL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	87-104%	95-105%	89-105%
EDTA plasma(n=5)	82-101%	84-100%	92-101%
heparin plasma(n=5)	83-98%	85-97%	80-92%

Sensitivity

< 46.875pg/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

RANKL/TNFSF11/CD254/ODF/OPGL/RANK L/TNFSF11/CD254 antigen/OPGLOPTB2/TRANCEODFhRANKL2

Notes

For Research Use Only

UniProt ID	O14788
Antigen	TNFSF11/RANKL

Applications	ELISA
Applications Notes	TNFSF11/RANKL ELISA Kit allows for the in vitro quantitative determination of TNFSF11/RANKL concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

Reference	Journal: Exerc Sci.2017; 26(1): 87-95. Title: The Effects of Exercise Intensity Difference on Bone Metabolic Markers and Cytokines of the RANKL/RANK/OPG System in Korean Osteopenia Elderly Women

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SKU	Supplier No.	Size	Your price	Quantity
BHE10806828	EH0313	96T	$400		Add to Cart

Human TNFSF11/RANKL(Tumor necrosis factor ligand superfamily member 11) ELISA Kit

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