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Home / ELISA Kit

Human Toll-like receptor 1 (TLR1) ELISA Kit

SKU : BHE13702535

TLR 1 is a member of the Toll-like receptor family (TLR) of pattern recognition receptors of the innate immune system. TLR1 recognizes pathogen-associated molecular pattern with a specificity for gram-positive bacteria. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is ubiquitously expressed, and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for this gene.

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Mfr.No.	KTE60317
Description	This Human Toll-like receptor 1 (TLR1) ELISA Kit employs a two-site sandwich ELISA to quantitate TLR1.
Alternative Names	TLR1; CD281; DKFZp547I0610; DKFZp564I0682; KIAA0012; MGC104956; MGC126311; MGC126312; TIL; rsc786; Toll/interleukin-1 receptor-like
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Toll-like receptor 1 microplate Human Toll-like receptor 1 standard Human Toll-like receptor 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Toll-like receptor 1 (TLR1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TLR1. No significant cross-reactivity or interference between Human TLR1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TLR 1 is a member of the Toll-like receptor family (TLR) of pattern recognition receptors of the innate immune system. TLR1 recognizes pathogen-associated molecular pattern with a specificity for gram-positive bacteria. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is ubiquitously expressed, and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for this gene.
Notes	This Human Toll-like receptor 1 (TLR1) ELISA Kit employs a two-site sandwich ELISA to quantitate TLR1 in samples. An antibody specific for TLR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTLR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TLR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	7096
Antigen	TLR1
Tag	TLR1
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Toll-like receptor 1 (TLR1) ELISA Kit employs a two-site sandwich ELISA to quantitate TLR1 in samples. An antibody specific for TLR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTLR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TLR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702535	KTE60317	48 T	$339		Add to Cart
BHE13702535	KTE60317	96 T	$589		Add to Cart
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