iNOS Antibody

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SKU

BHA12902884
Dilution

WB: 1:500~1:3000 IHC: 1:50~1:200 IF/ICC: 1:100~1:500, ELISA(peptide) 1:20000-1:40000
Applications

IF/ICC, ELISA, IHC, WB
Alternative Names

HEP-NOS|Hepatocyte NOS|HEPNOS|inducible|Inducible nitric oxide synthase|Inducible NO synthase|Inducible NOS|iNOS|MAC NOS|Macrophage NOS|Nitric...

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$260

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Mfr.No.

AF0199
Dilution

WB: 1:500~1:3000 IHC: 1:50~1:200 IF/ICC: 1:100~1:500, ELISA(peptide) 1:20000-1:40000
Applications

IF/ICC, ELISA, IHC, WB

Alternative Names

HEP-NOS|Hepatocyte NOS|HEPNOS|inducible|Inducible nitric oxide synthase|Inducible NO synthase|Inducible NOS|iNOS|MAC NOS|Macrophage NOS|Nitric oxide synthase 2 inducible|Nitric oxide synthase 2 inducible macrophage|nitric oxide synthase 2A (inducible, hepatocytes)|Nitric oxide synthase|Nitric oxide synthase inducible|nitric oxide synthase, macrophage|NOS 2|NOS|Nos II|NOS type II|nos2|NOS2_HUMAN|NOS2A|NOS2A, Inducible, Hepatocyte|Peptidyl-cysteine S-nitrosylase NOS2|
Categories

Primary Antibodies
Description

iNOS Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Belongs to the NOS family. Induced by endotoxins and cytokines. Induced by interferon gamma acting synergistically with lipopolysaccharide, tumor necrosis factor alpha or interleukin-1 beta. Homodimer. Binds SLC9A3R1. 2 isoforms of the human protein are produced by alternative splicing.
Molecular Weight

Observed Mol.Wt.: 130kDa.
Predicted Mol.Wt.: 132kDa.
Specificity

iNOS antibody detects endogenous levels of total iNOS.
Antigen

iNOS
Clonality

polyclonal
Immunogen

A synthesized peptide derived from human iNOS.
Reactivity

Human, Mouse, Rat

Concentration

1mg/ml
Purification

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage Condition

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Format

Liquid

Gene Id

4843
Uniprot

P35228

Sequence

10	20	30	40	50
MACPWKFLFK	TKFHQYAMNG	EKDINNNVEK	APCATSSPVT	QDDLQYHNLS
60	70	80	90	100
KQQNESPQPL	VETGKKSPES	LVKLDATPLS	SPRHVRIKNW	GSGMTFQDTL
110	120	130	140	150
HHKAKGILTC	RSKSCLGSIM	TPKSLTRGPR	DKPTPPDELL	PQAIEFVNQY
160	170	180	190	200
YGSFKEAKIE	EHLARVEAVT	KEIETTGTYQ	LTGDELIFAT	KQAWRNAPRC
210	220	230	240	250
IGRIQWSNLQ	VFDARSCSTA	REMFEHICRH	VRYSTNNGNI	RSAITVFPQR
260	270	280	290	300
SDGKHDFRVW	NAQLIRYAGY	QMPDGSIRGD	PANVEFTQLC	IDLGWKPKYG
310	320	330	340	350
RFDVVPLVLQ	ANGRDPELFE	IPPDLVLEVA	MEHPKYEWFR	ELELKWYALP
360	370	380	390	400
AVANMLLEVG	GLEFPGCPFN	GWYMGTEIGV	RDFCDVQRYN	ILEEVGRRMG
410	420	430	440	450
LETHKLASLW	KDQAVVEINI	AVLHSFQKQN	VTIMDHHSAA	ESFMKYMQNE
460	470	480	490	500
YRSRGGCPAD	WIWLVPPMSG	SITPVFHQEM	LNYVLSPFYY	YQVEAWKTHV
510	520	530	540	550
WQDEKRRPKR	REIPLKVLVK	AVLFACMLMR	KTMASRVRVT	ILFATETGKS
560	570	580	590	600
EALAWDLGAL	FSCAFNPKVV	CMDKYRLSCL	EEERLLLVVT	STFGNGDCPG
610	620	630	640	650
NGEKLKKSLF	MLKELNNKFR	YAVFGLGSSM	YPRFCAFAHD	IDQKLSHLGA
660	670	680	690	700
SQLTPMGEGD	ELSGQEDAFR	SWAVQTFKAA	CETFDVRGKQ	HIQIPKLYTS
710	720	730	740	750
NVTWDPHHYR	LVQDSQPLDL	SKALSSMHAK	NVFTMRLKSR	QNLQSPTSSR
760	770	780	790	800
ATILVELSCE	DGQGLNYLPG	EHLGVCPGNQ	PALVQGILER	VVDGPTPHQT
810	820	830	840	850
VRLEALDESG	SYWVSDKRLP	PCSLSQALTY	FLDITTPPTQ	LLLQKLAQVA
860	870	880	890	900
TEEPERQRLE	ALCQPSEYSK	WKFTNSPTFL	EVLEEFPSLR	VSAGFLLSQL
910	920	930	940	950
PILKPRFYSI	SSSRDHTPTE	IHLTVAVVTY	HTRDGQGPLH	HGVCSTWLNS
960	970	980	990	1000
LKPQDPVPCF	VRNASGFHLP	EDPSHPCILI	GPGTGIAPFR	SFWQQRLHDS
1010	1020	1030	1040	1050
QHKGVRGGRM	TLVFGCRRPD	EDHIYQEEML	EMAQKGVLHA	VHTAYSRLPG
1060	1070	1080	1090	1100
KPKVYVQDIL	RQQLASEVLR	VLHKEPGHLY	VCGDVRMARD	VAHTLKQLVA
1110	1120	1130	1140	1150
AKLKLNEEQV	EDYFFQLKSQ	KRYHEDIFGA	VFPYEAKKDR	VAVQPSSLEM

SAL

Manual

(pdf)
MSDS
IHC Protocol.
Western Blotting Handbook.

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iNOS Antibody

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