Mouse Anti-KDEL Monoclonal IgG1

Mfr.No.	SMC-538, SMC-538D, SMC-538D-A390, SMC-538D-A488, SMC-538D-A594, SMC-538D-ALP, SMC-538D-APC, SMC-538D-BI, SMC-538D-FITC, SMC-538D-HRP, SMC-538D-PCP, SMC-538D-RPE, SMC-538S
Alternative Names	KDEL Antibody, Lys Asp Glu Leu Antibody, Lys-Asp-Glu-Leu (KDEL) Antibody, Lys-Asp-Glu-Leu Antibody, Lysine-aspartic acid-glutamate-leucine Antibody
Antigen	KDEL
Categories	Antibodies
Certificate Of Analysis	A 1:1000 dilution of SMC-538 was sufficient for detection of KDEL in 10 ug of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Clonality	Monoclonal
Conjugate	Unconjugated, ATTO 390, ATTO 488, ATTO 594, Alkaline Phosphatase, APC, Biotin, FITC, HRP, PerCP, RPE
Description	Mouse Anti-KDEL Monoclonal IgG1
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)
Immunogen Region	KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)
Isotype	IgG1
Launch Date	2017/10/10
Net Weight (g)	0.1
Reactivity	Rat, Mouse, Human
Recombinant	FALSE
Regulatory	RUO
Species Reactivity Medium	Mouse, Rat, Human

Target	KDEL
Clone No	1F2
Research Area	Cell Signaling, Tags and Cell Markers, Organelle Markers, Chaperone Proteins, Protein Trafficking

Buffer	PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration	1 mg/ml
Dilutions	WB (1:1000); optimal dilutions for assays should be determined by the user.
Purification	Protein G Purified
Scientific Background	The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects KDEL tagged proteins.
Storage	-20ºC
Storage Buffer	PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	WB
Description	WB
Dilution	WB (1:1000); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Ozawa K., et al. (2008) Mol Pharmacol. 74:1610. 2. Austin R.C., et al. (2003) J Biol Chem. 278: 17438.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11905882-1	SMC-538D	Unconjugated	100 ug	$330	Add to Cart
BHA11905882-2	SMC-538D-A390	ATTO 390	100 ug	$384	Add to Cart
BHA11905882-3	SMC-538D-A488	ATTO 488	100 ug	$383	Add to Cart
BHA11905882-4	SMC-538D-A594	ATTO 594	100 ug	$383	Add to Cart
BHA11905882-5	SMC-538D-ALP	Alkaline Phosphatase	100 ug	$376	Add to Cart
BHA11905882-6	SMC-538D-APC	APC	100 ug	$382	Add to Cart
BHA11905882-7	SMC-538D-BI	Biotin	100 ug	$378	Add to Cart
BHA11905882-8	SMC-538D-FITC	FITC	100 ug	$374	Add to Cart
BHA11905882-9	SMC-538D-HRP	HRP	100 ug	$370	Add to Cart
BHA11905882-10	SMC-538D-PCP	PerCP	100 ug	$382	Add to Cart
BHA11905882-11	SMC-538D-RPE	RPE	100 ug	$380	Add to Cart
BHA11905882-12	SMC-538S	Unconjugated	12 ug	$44	Add to Cart

KDEL Antibody, Clone 1F2 - SMC-538