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Home / Biochemicals / Small Molecules

Leptomycin B

SKU : BHB21300213

A Protein Synthesis and Transport Inhibitor

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Alomone Labs

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Mfr.No.	L-500, L-500_1 mcg, L-500_5 mcg
Description	Leptomycin B (#L-500) is a highly pure, natural, and biologically active compound.
Molecular Formula	C33H48O6.
Synonyms	Natural
Background	Leptomycin B is an unsaturated, branched-chain fatty acid antibiotic which produced by Streptomyces sp. ATS1287.1,2 Leptomycin B was found to act as an antifungal antibiotic and reversible antiproliferative agent which arrests the cell cycle in both the G1 and G2 phases into the resting state - GO.3In the late 90's Leptomycin B, was found as inhibitor of the nucleo-cytoplasmic translocation process of the HIV-1 Rev protein and Rev-dependent mRNA at nanomolar concentrations and was the first low molecular weight inhibitor of nuclear export to be identified.4The target molecule of Leptomycin B has been shown by genetic and biochemical analyses to be CRM1, a highly conserved protein in eukaryotes. CRM1 was shown to be a member of the importin beta family and a receptor for the nuclear export signal (NES) of proteins in both yeast and mammalian cells. Leptomycin B binds directly to CRM1, which results in the dissociation of the NES from the nuclear export machinery containing CRM1 and leads to the nuclear accumulation of many proteins in both yeast and mammalian cells.5-7Thus, Leptomycin B serves as a potent tool for understanding the molecular mechanisms of nucleo-cytoplasmic transport of proteins.
Shipping	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Concentration	1-50 nM.
Reconstitution	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeat freeze-thawing may result in loss of activity.
Cas Number	87081-35-4
Molecular Weight	540.75
Solubility	Product is supplied in 100 ul 70% methanol (1 ug supplied at a concentration of 18.5 uM, and 5 ug supplied at a concentration of 92.5 uM). Keep on ice when working to prevent solvent evaporation. Do not dry. Do not use DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Activity	Leptomycin B, an unsaturated, branched fatty-acid chain, is an antifungal antibiotic1,2 which arrests the cell cycle3 and prevents the nuclear export of proteins4,5.
Country Of Origin	Israel/IL
UNSPSC Code	41116134
Application	FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay
Lead Time	1-2 Business Days
Notes	Bioassay Tested: Yes Chemical Name: (2E,5S,6R,7S,9R,10E,12E,15R,16Z,18E)-19-((2S,3S)-3,6-Di hydro-3-methyl-6-oxo-2H-pyran-2-yl)-17-ethyl-6-hydroxy-3,5,7,9,11,15-hexamethyl-8-oxo-2,10,12,16,18 nonadecapentaenoic acid.

Purity	>98%
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. Storage of solutions: Store at -20°C. Protect from light.

Product Manual	SDS

Reference	Hamamoto, T. et al. (1983) J. Antibiot. (Tokyo) 36, 639. Hamamoto, T. et al. (1985) J. Antibiot. (Tokyo) 38, 1573. Yoshida, M. et al. (1990) Exp. Cell Res. 187, 150. Wolff, B. et al. (1997) Chem. Biol. 4, 139. Ossareh-Nazari, B. et al. (1997) Science 278, 141. Fornerod, M. et al. (1997) Cell 90, 1051. Kudo, N. et al. (1998) Exp. Cell Res. 242, 540. Hamamoto, T. et al. (1983) J. Antibiot. (Tokyo) 36, 639. Hamamoto, T. et al. (1985) J. Antibiot. (Tokyo) 38, 1573. Yoshida, M. et al. (1990) Exp. Cell Res. 187, 150. Wolff, B. et al. (1997) Chem. Biol. 4, 139. Kudo, N. et al. (1998) Exp. Cell Res. 242, 540.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHB21300213-1	L-500_1 mcg	1 mcg	$55		Add to Cart
BHB21300213-2	L-500_5 mcg	5 mcg	$188		Add to Cart
BHB21300213-3	L-500_1 mcg	5x1 mcg	$206		Add to Cart

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