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Liposome Plasma Stability Test Kit

BHE16300408

This product includes 20 spin-columns for liposome stability studies in plasma

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Contact us to order
Tel
+1 866.986.9598
Email
[email protected]
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Product Manual Datasheet
Protocols Description PROTOCOL
1. Column preparation
(1) Remove the caps of 1.5-ml Eppendorf tubes and use them as receiver tubes. Remove the bottom tip and the cap of each spin column and insert the column into a receiving tube.
(2) Spin the columns at 1000 rpm using a benchtop Eppendorf centrifuge for 1 min and discard the elute.
(3) Spin the columns at 1000 rpm for 4 min and change to a clean receiving tube.
 
2. Sample preparation
(1) Filtrate human plasma through a 0.22 µm syringe filter (low protein binding filter, Pall Scientific, PN 4902).
(2) Mix 100 µl of liposome and 100 µl of plasma and incubate at 37 °C for 2 to 24 hr.
(3) Mix 100 µl of liposome and 100 µl of buffer and keep it on ice as a control.
 
3. Separation of the intact liposomal drug from the free and protein-bound drugs
(1) Load 75 µl of the plasma-treated liposome sample or control onto each column.
(2) Spin the column at 1000 rpm for 4 min. Collect the elute. Discard the column.
 
4. Analysis of Liposome stability in plasma
(1) Use a proper method to measure the drug concentrations in the elute of the plasma-treated liposome sample (Cp) and the elute of the control (Cc).
(2) Analyze the stability of the liposome in plasma:
Recovery yield of intact liposome in plasma (%) = Cp x 100 % / Cc
SKU Supplier No. Size Your price Quantity
BHE16300408 SPS20 20 columns $293.74
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