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Home / Primary Antibodies / Monoclonal Antibodies

beta-Tubulin Antibody

SKU : BHA12907944

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Tubulin may not be stable in certain cells. For example, expression of beta-Tubulin in adipose tissue is very low and therefore beta-Tubulin should not be used as loading control for these tissues.

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AFFINITY BIOSCIENCES LTD

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Mfr.No.	T0023
Alternative Names	TUBB3, CDCBM, Beta III Tubulin, Class III beta-tubulin, TUBB4, Tubulin, beta 3, Tubulin beta-III, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin, beta 3 class III, CFEOM3A
Antigen	beta-tubulin
Categories	Primary Antibodies
Clonality	monoclonal
Description	Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Tubulin may not be stable in certain cells. For example, expression of beta-Tubulin in adipose tissue is very low and therefore beta-Tubulin should not be used as loading control for these tissues.
Immunogen	Full-length beta-tubulin protein of human.
Molecular Weight	Observed Mol.Wt.: 55KD. Predicted Mol.Wt.: 50kDa.
Reactivity	Chicken, Hamster, Monkey, Rabbit, Human, Mouse, Sheep, Dog, Rat
Specificity	beta-Tubulin Mouse Monoclonal antibody detects endogenous levels of total beta-Tubulin protein.
Subcellular Location	Cytoplasm > cytoskeleton.

Uniprot

P07437

Gene Id

203068

Sequence

10	20	30	40	50
MREIVHIQAG	QCGNQIGAKF	WEVISDEHGI	DPTGTYHGDS	DLQLDRISVY
60	70	80	90	100
YNEATGGKYV	PRAILVDLEP	GTMDSVRSGP	FGQIFRPDNF	VFGQSGAGNN
110	120	130	140	150
WAKGHYTEGA	ELVDSVLDVV	RKEAESCDCL	QGFQLTHSLG	GGTGSGMGTL
160	170	180	190	200
LISKIREEYP	DRIMNTFSVV	PSPKVSDTVV	EPYNATLSVH	QLVENTDETY
210	220	230	240	250
CIDNEALYDI	CFRTLKLTTP	TYGDLNHLVS	ATMSGVTTCL	RFPGQLNADL
260	270	280	290	300
RKLAVNMVPF	PRLHFFMPGF	APLTSRGSQQ	YRALTVPELT	QQVFDAKNMM
310	320	330	340	350
AACDPRHGRY	LTVAAVFRGR	MSMKEVDEQM	LNVQNKNSSY	FVEWIPNNVK
360	370	380	390	400
TAVCDIPPRG	LKMAVTFIGN	STAIQELFKR	ISEQFTAMFR	RKAFLHWYTG
410	420	430	440
EGMDEMEFTE	AESNMNDLVS	EYQQYQDATA	EEEEDFGEEA	EEEA

Concentration	1mg/ml
Format	Liquid
Purification	Affinity-chromatography.
Storage Condition	Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl.

Applications	IF/ICC, ELISA, IHC, WB
Dilution	WB: 1:10000~1:500000, IHC: 1:200, IF/ICC: 1:200, ELISA(peptide) 1:20000-1:40000

Manual
MSDS
IHC Protocol.
Western Blotting Handbook.

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SKU	Supplier No.	Size	Your price	Quantity
BHA12907944	T0023	100ul	$250		Add to Cart
BHA12907944	T0023	1ml	$1200		Add to Cart
BHA12907944	T0023	200ul	$350		Add to Cart
BHA12907944	T0023	50ul	$150		Add to Cart

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