WB: 1/500 - 1/5000, IF/ICC: 1/20 - 1/200. Optimal dilutions/concentrations should b e determined by the end user.
Description
MTOR (pS2481) Antibody is a Monoclonal Antibody against MTOR.
Immunofluorescence staining of Hela cells using MTOR (pS2481) Antibody at 1/100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?C. The secondary antibody was AF488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).