T4 RNA Ligase 1

T4 RNA Ligase 1, also known as T4 RNA ligase, is an ATP-dependent enzyme that catalyzes the formation of a phosphodiester bond between the 5'-P terminus and the 3'-OH terminus of single-stranded RNA (ssRNA), single-stranded DNA (ssDNA), or mononucleotide molecules. T4 RNA Ligase 1 can perform ligation between ssRNA molecules (most efficiently), between ssDNA and ssRNA (with lower efficiency), and can also be used for ligation between ssDNA molecules (with very low efficiency). It is suitable for the ligation of ssRNA molecules and for the attachment of 5' adapters in miRNA library construction for NGS.

Figure 1. Mechanism of Action of T4 RNA Ligase 1

Features

Catalyzes the formation of a phosphodiester bond between the 5'-P terminus and the 3'-OH terminus of single-stranded RNA (ssRNA), single-stranded DNA (ssDNA), or mononucleotide molecules.

Applications

RNA Cloning

5' adapters in miRNA library construction for NGS

Circular RNA Preparation

Specifications