T4 RNA Ligase 2 (10 U/uL)

T4 RNA Ligase 2, also known as T4 RNA ligase 2, is an ATP-dependent double-stranded RNA ligase that exhibits both inter- and intra molecular ligation activities on RNA strands. Compared to T4 RNA Ligase 1, this enzyme displays a significantly higher activity in ligating nicks in double-stranded RNA than in ligating single-stranded RNA ends. The proximity of a 5'-phosphate group and a 3'-hydroxyl group is required for ligation. Moreover, it has the unique capability to ligate between the 3´-hydroxyl group of RNA and the 5´-phosphate group of DNA within a double-stranded structure.

Features

Strong ligation activity: capable of effectively linking dsRNA substrates.

No residual nuclease, nicking enzyme, or RNase.

Applications

Connecting the cleavage site of dsRNA.

Connecting the 3´ hydroxyl group of RNA to the 5´ phosphate group of double-stranded DNA structure.

Circular RNA preparation.

Specifications

Components

Shipping and Storage

This product should be stored at -25 ~ -15^oC for 1 years.

Figures

Figure 1. Verification of T4 RNA ligase 2 ligation efficiency.

Note: The 20 ?L reaction system contains dsRNA ligation substrates at a final concentration of 20 ?M.

Documents:      

Safety Data Sheet

14652_MSDS_Ver.EN20250121.pdf

Manuals

14652_Manual_Ver.EN20250121.pdf